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Application of liquid biopsy in precision medicine: opportunities and challenges
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《医学前沿(英文)》 2017年 第11卷 第4期 页码 522-527 doi: 10.1007/s11684-017-0526-7
Precision medicine for cancer patients aims to adopt the most suitable treatment options during diagnosis and treatment of individuals. Detecting circulating tumor cell (CTC) or circulating tumor DNA (ctDNA) in plasma or serum could serve as liquid biopsy, which would be useful for numerous diagnostic applications. Liquid biopsies can help clinicians screen and detect cancer early, stratify patients to the most suitable treatment and real-time monitoring of treatment response and resistance mechanisms in the tumor, evaluate the risk for metastatic relapse, and estimate prognosis. We summarized the advantages and disadvantages of tissue and liquid biopsies. We also further compared and analyzed the advantages and limitations of detecting CTCs, ctDNAs, and exosomes. Furthermore, we reviewed the literature related with the application of serum or plasma CTCs, ctDNAs, and exosomes for diagnosis and prognosis of cancer. We also analyzed their opportunities and challenges as future biomarkers. In the future, liquid biopsies could be used to guide cancer treatment. They could also provide the ideal scheme to personalize treatment in precision medicine.
关键词: liquid biopsy circulating tumor cells cell-free ctDNA exosomes precision medicine
Preliminary study on preparation of cell-free system for protein expression
CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua
《化学科学与工程前沿(英文)》 2008年 第2卷 第2期 页码 224-229 doi: 10.1007/s11705-008-0029-9
关键词: cell-free regeneration extract information successful construction
Cell-free systems in the new age of synthetic biology
Fernando Villarreal,Cheemeng Tan
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 58-65 doi: 10.1007/s11705-017-1610-x
Microcystin-LR biodegradation by
Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN
《环境科学与工程前沿(英文)》 2011年 第5卷 第4期 页码 526-532 doi: 10.1007/s11783-010-0261-7
关键词:
microcystin-LR(MC-LR)
biodegradation
通过蛋白质工程提高聚酯水解酶对PET 塑料的降解效率 Article
马渊, 姚明东, 李炳志, 丁明珠, 何博, 陈思, 周晓, 元英进
《工程(英文)》 2018年 第4卷 第6期 页码 888-893 doi: 10.1016/j.eng.2018.09.007
来自Ideonella sakaiensis 的聚对苯二甲酸乙二醇酯水解酶(PETase)在室温下具有很强的降解聚对苯二甲酸乙二醇酯(PET)的能力,因此被认为是解决聚酯塑料污染问题的潜在工具。在本研究中,基于PETase 和底物2PET 结合时的相互作用模型,分析了PETase 与底物2PET 之间的相互作用,从而对底物结合沟壑周围的六个关键残基进行改造。为了更加快速地筛选突变体酶的活性,本研究利用无细胞蛋白表达体系对设计的PETase 突变体进行高通量的表达和验证。最终发现三种突变体(R61A、L88F 和I179F)的酶活性相比野生型分别提高了1.4 倍、2.1 倍和2.5 倍。其中,I179F突变体的酶活性最高,降解效率为22.5 mg·μmol–1·d–1。因此,本研究通过蛋白质工程对PETase 的关键疏水位点进行设计和改造,获得了降解效果提高的酶突变体,并进一步证实了其生物降解塑料的潜力。
6G广域时延敏感物联网——移动边缘计算使能的非地面网络设计 Article
刘承骁, 冯伟, 陶晓明, 葛宁
《工程(英文)》 2022年 第8卷 第1期 页码 96-107 doi: 10.1016/j.eng.2021.11.002
在即将到来的第六代移动通信技术(6G)时代,广域时延敏感物联网(IoT)需求不断增加。由于传统蜂窝技术难以直接用于广域时延敏感IoT,因此使用非地面基础设施,包括卫星和无人机(UAV)等,基于无蜂窝网络架构构建非地面网络(NTN)亟待研究。考虑到时延敏感业务需求和IoT 设备分布不均的特点,NTN可使用移动边缘计算(MEC)来增强业务支持能力,并提供“沙漠绿洲”状(oasis-oriented)的按需覆盖。然而,在MEC使能的NTN中,通信和MEC系统相互耦合,带来通信与计算资源协同编排难题。本文提出一种面向过程的优化框架,在更大时间尺度上设计通信和MEC系统。在这个框架中,本研究采用大尺度信道状态信息(CSI)刻画复杂传播环境,构建通信与计算资源联合编排的时延最小化问题;给出该优化问题的近似问题,并将其分解为多个子问题;然后,提出迭代算法来求解这些子问题。仿真结果表明,与现有算法相比,所提出的面向过程的方案可以降低任务总时延。这为无人机载荷部署提供了依据,也显示了在广域时延敏感IoT中NTN与MEC协同设计的潜力。
An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis
M. H. NGUYEN-THOI,L. Le-ANH,V. Ho-HUU,H. Dang-TRUNG,T. NGUYEN-THOI
《结构与土木工程前沿(英文)》 2015年 第9卷 第4期 页码 341-358 doi: 10.1007/s11709-015-0302-1
关键词: cracked Reissner-Mindlin shell free vibration analysis cell-based smoothed discrete shear gap method (CS-FEM-DSG3) extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) smoothed finite element methods (SFEM)
NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis
Gang WANG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 1-7 doi: 10.1007/s11684-009-0018-5
关键词: free radicals tumor phox cell proliferation cancer therapy
null
《医学前沿(英文)》 2018年 第12卷 第2期 页码 153-163 doi: 10.1007/s11684-017-0548-1
To establish optimal reference values for recovered immune cell subsets, we prospectively investigated post-transplant immune reconstitution (IR) in 144 patients who received allogeneic stem cell transplantation (allo-SCT) and without showing any of the following events: poor graft function, grades II?IV acute graft-versus-host disease (GVHD), serious chronic GVHD, serious bacterial infection, invasive fungal infection, or relapse or death in the first year after transplantation. IR was rapid in monocytes, intermediate in lymphocytes, CD3+ T cells, CD8+ T cells, and CD19+ B cells, and very slow in CD4+ T cells in the entire patient cohort. Immune recovery was generally faster under HLA-matched sibling donor transplantation than under haploidentical transplantation. Results suggest that patients with an IR comparable to the reference values display superior survival, and the levels of recovery in immune cells need not reach those in healthy donor in the first year after transplantation. We suggest that data from this recipient cohort should be used as reference values for post-transplant immune cell counts in patients receiving HSCT.
关键词: immune reconstitution hematopoietic stem cell transplantation event-free patients reference range
The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,
Xinxin LI,Huijuan WANG,Guanghua SU,Zhuying WEI,Chunling BAI,Wuni-MENGHE,Yanhui HOU,Changqing YU,Shorgan BOU,Guangpeng LI
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 191-200 doi: 10.15302/J-FASE-2014029
关键词: somatic cell nuclear transfer free-grazing synchronization Dorper sheep cloned animal ecology
Minimal residual disease in solid tumors: an overview
《医学前沿(英文)》 2023年 第17卷 第4期 页码 649-674 doi: 10.1007/s11684-023-1018-6
关键词: MRD solid tumor CTC ctDNA
Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell
Alireza AHMADIAN YAZDI, Jie XU
《能源前沿(英文)》 2018年 第12卷 第2期 页码 233-238 doi: 10.1007/s11708-018-0529-3
关键词: enzymatic fuel cell nitrogen-doped graphene reduced graphene oxide catalyst-free synthesis
Xuejiao Wang, Xiang Feng, Jing Shang
《环境科学与工程前沿(英文)》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1061-8
•The efficient PEC degradation of RhB is realized using no photocatalyst. •The efficient PEC degradation of RhB features the low salinity. •The PEC degradation of RhB takes place on the anode and cathode simultaneously.
关键词: Energy relay structure Energy saving Photocatalyst-free and low-salinity degradation Photoelectrochemical cell
《化学科学与工程前沿(英文)》 2023年 第17卷 第12期 页码 1879-1894 doi: 10.1007/s11705-023-2354-4
关键词: carbon dioxide halogen-free catalysis cyclic carbonate mechanistic insight
Hollow carbon spheres and their noble metal-free hybrids in catalysis
《化学科学与工程前沿(英文)》 2021年 第15卷 第6期 页码 1380-1407 doi: 10.1007/s11705-021-2097-z
关键词: hollow carbon spheres functionalization noble metal-free catalysis
标题 作者 时间 类型 操作
Preliminary study on preparation of cell-free system for protein expression
CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua
期刊论文
Microcystin-LR biodegradation by
Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN
期刊论文
An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis
M. H. NGUYEN-THOI,L. Le-ANH,V. Ho-HUU,H. Dang-TRUNG,T. NGUYEN-THOI
期刊论文
Comparison of reference values for immune recovery between event-free patients receiving haploidentical
null
期刊论文
The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,
Xinxin LI,Huijuan WANG,Guanghua SU,Zhuying WEI,Chunling BAI,Wuni-MENGHE,Yanhui HOU,Changqing YU,Shorgan BOU,Guangpeng LI
期刊论文
Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell
Alireza AHMADIAN YAZDI, Jie XU
期刊论文
Efficient photoelectrochemical oxidation of rhodamine B on metal electrodes without photocatalyst or supporting electrolyte
Xuejiao Wang, Xiang Feng, Jing Shang
期刊论文
Recent advances in cycloaddition of CO with epoxides: halogen-free catalysis and mechanistic insights
期刊论文